欧美另类视频-欧美另类性-欧美另类杂交a-欧美另类重口-欧美另类专区-欧美乱妇

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-27842V6.11 JHU-67 人胚腎細(xì)胞

2V6.11 JHU-67 人胚腎細(xì)胞

簡(jiǎn)要描述:CRL-2784 2V6.11 JHU-67 人胚腎細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,。細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-2784
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:1727

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-2784 2V6.11 JHU-67 人胚腎細(xì)胞

ATCC® Number:  CRL-2784™

Designations:  2V6.11 [JHU-67]

Depositors:   G Ketner

Biosafety Level: 2 [Cells containing Adenovirus viral DNA sequences ]

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

CRL-2784 2V6.11 JHU-67 人胚腎細(xì)胞

Source: Organ: kidney

Cell Type: epithelialtransformed with adenovirus 5 DNA

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Restrictions: Part of the Johns Hopkins Special Collection

Isolation:  Isolation date: 2001

Age:  fetus

Comments: This line was derived from the human embryonic kidney line, 293 in 2001. 293 cells were transfected with the plasmid pVgRXR bearing a Zeocin-resistance selectable marker to obtain 293VgRXR cells. This population was subsequently transfected with the plasmid pEKORF6 containing Ad5 E4 ORF 6 that encodes E4 34K. pEKORF6 is a derivative of the plasmid pIndHydro that contains a hygromycin resistance gene. The vectors contain SV40 viral DNA sequences. The 2V6.11 cell line was originally selected in hygromycin-containing medium and cloned by single-colony isolation with a cloning cylinder. The 2V6.11 cells inducibly express the human adenovirus E4, 34kDa protein (E4 ORF6). 2V6.11 cells exhibit little or no constitutive E4 biologic activity. Induction by ponasterone A, an ecdysone analog, however, results in E4 biologic activity and levels of E4 34kDa protein that are detectable by immunoblotting. These cells can be used as a tool to study the biology of the adenoviral E4 oncoprotein.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: The depositor recommends periodic hygromycin selection.

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

An inoculum of 5 X 10(3) to 1 X 10(4) viable cells/cm2 is recommended.

Incubate cultures at 37°C.

Interval: Maintain cultures at a cell concentration between 2 X 10(4) and 2 X 10(5) cells/cm2.

Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended

Medium Renewal: Two to three times weekly

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time:  23 hrs.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

source culture:ATCC JHU-67

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products


CRL-2784



















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:[email protected]

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 国产成人户外露出视频在线观看 | 国产一区二区韩国一区二区日本一区二区 | 国内精品A片XXX久久久 | 国产高清无码中文 | 成人免费无码精品国产电影同人 | 久久久久精品国产熟女影院 | 久久久久精品国产三级 | 欧洲国产成人综合一本 | 欧美三级网站高清国产不卡 | 国内精品久久久久久久影视 | 亚洲vs日韩vs欧美vs久久 | 青青草原综合久久大伊人精品 | 91人人揉日日捏人人看 | 国产成人精品一区二区三区在线 | 国产一区二区三区四区精华 | 国产成人女人在线观看 | 亚洲欧美国产精 | 精品国产综合成人亚洲区 | 亚洲精品久久久一区二区三区 | 国产色综合色产在线视频 | 精品国产无限资源免费观看 | 国产精品人人 | 2024精品国产自在现线 | 欧美日韩精品一区二区在线播放 | 日韩一级中文字幕在线 | 男插女爽到内射的视频 | 高清国产美女一级a毛片在线 | jizz一日本日韩片 | 伊人久久久综在合线久久在播 | 日产精品一二三四区气温 | 国产午夜福利短视频在线观看 | 国产精品青青在线一区 | 99国产精品农村一级毛片 | 精品视频在线观看你懂的一区 | 国产亚洲tv在线观看 | 国产二区视频在线播放蜜臀 | 精品久久久久久无码中文字幕一 | 91精品国产色综合久久不卡 | 亚洲欧美综合一区二区三区黄大片 | 国产经典一区二区三区蜜芽 | 国产熟女免费观看久久黄av片 |